Biological Nitrogen Removal Database

Castellaniella caeni

• Phylum : Proteobacteria
• Class : Betaproteobacteria
• Order :Burkholderiales
• Family : Alcaligenaceae
• Genus : Castellaniella

NO₃^-

Glucose

Reference:Liu et al., 2008

Title:Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant 

Pubmed ID:18768620

Pubmed link:Link

Full research link:Link

Abstract: A Gram-negative, non-motile, facultatively anaerobic, denitrifying bacterial strain, designated Ho-11T, was isolated from sludge of a leachate treatment plant and was characterized taxonomically by using a polyphasic approach. The G+C content of the genomic DNA was 63.5 mol%. Strain Ho-11T contained ubiquinone Q-8 as the major respiratory lipoquinone and putrescine as the predominant polyamine. The major fatty acids were summed feature 4 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH; 29.3 %), C16 : 0 (28.0 %) and summed feature 7 (C18 : 1 ω7c, C18 : 1 ω9t and/or C18 : 1 ω12t; 19.8 %). Comparative 16S rRNA gene sequence analysis showed that strain Ho-11T belonged to the family Alcaligenaceae, class Betaproteobacteria, and joined the evolutionary radiation enclosed by the genus Castellaniella. 16S rRNA gene sequence similarities between strain Ho-11T and the type strains of the two recognized species of the genus, Castellaniella denitrificans DSM 11046T and Castellaniella defragrans DSM 12141T, were 97.8 and 97.4 %, respectively. Levels of similarity between strain Ho-11T and all other recognized species of the family Alcaligenaceae were below 95.6 %. Strain Ho-11T exhibited relatively low levels of DNA–DNA relatedness with respect to C. denitrificans DSM 11046T (33 %) and C. defragrans DSM 12141T (28 %). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain Ho-11T (=KCTC 12197T=LMG 23411T) should be classified in the genus Castellaniella as the type strain of a novel species, for which the name Castellaniella caeni sp. nov. is proposed.